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Pharmaceutical Application Notes

Gilson, Inc., a global leader in preparative HPLC, offers a full line of chromatographic instruments to suit the needs of today’s scientist in the purification market. From robust instruments for reformatting techniques to complicated assay procedures with disposable tip or fixed probe options, Gilson has an automated solution to meet your liquid handling application requirements.

 

Purification of Vanillin by a Combination of Flash and Preparative HPLC with the PLC 2250

 

KEYWORDS: Vanillin, Vanilla Extract, PLC Purification Systems, FLASH Chromatography, Preparative HPLC.

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Streamlined Purification of Assay-ready Oligonucleotides by Automated HPLC

 

KEYWORDS: Oligonucleotides, qPCR, Next Generation Sequencing, Microarray Analysis, GX-271, Purification, TRILUTION LC

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Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System


 

KEYWORDS: High-Performance Liquid Chromatography (HPLC), Mass Spectrometry (MS), Preparative HPLC/MS, Fraction Collection, Fraction Reinjection, Probenecid, TRILUTION LC, Conditional Logic Fraction Collection

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Evaluation of Ketone-Containing Terpene Compounds Using Evaporative Light Scattering Detection


 

KEYWORDS: PrepELS™II Detector, Evaporative Light Scattering (ELS), High-Performance Liquid Chromatography (HPLC), Terpenes, Ketones, Thujone, Camphor, Essential Oil Constituents, Volatile Compounds

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Automating the Promega GoTaq® PCR Core System II Reaction Using the Gilson PIPETMAX® 268


 

KEYWORDS: Promega GoTaq PCR Core System II, PIPETMAX 268, Plasmid DNA

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High-Resolution Purification of Sheared Lambda Phage DNA Using a Gilson HPLC System


 

KEYWORDS: High-Performance Liquid Chromatography (HPLC), DNA Shearing, DNA Separation, Methylation, Lambda Phage, Gradient Pattern, Electrophoresis, Next-Generataion Sequencing (NGS)

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Automated Patient Barcode Tracking and Sample Preparation Using the Gilson GX-281 Barcode Matching System


 

KEYWORDS: GX-281 Liquid Handler, Sample Tracking, Barcode Matching, Laboratory Information Management System (LIMS), Laboratory Informatics, Sample Preparation, Automation, Clinical Laboratory, Patient Samples

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Recovery and Purification Comparison of Glycinin Using Various pH Extraction Procedures on a Continuous Injection Gradient Gilson PLC 2020 System


 

KEYWORDS: Glycinin, Soy Protein, Beta Conglycinin, PLC 2020 Personal Purification System, Semi-Preparative Purification, Extraction, HPLC, Continuous Injection, Large Volume Loading, Automated Loading Pump, Gradient

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Purification of Aglain Natural Products with the Gilson PLC 2020 Personal Purification System


 

KEYWORDS: Aglain, Natural Product HPLC Purification, Synthesis, PLC 2020

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Isolation of Synthetic Pleiomaltinine Using the Gilson PLC 2020 Personal Purification System


 

KEYWORDS: Pleomaltinine, Synthetic HPLC Purification, PLC 2020, Biologically Active, Pyrone

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Selecting the Proper Pipetting Tools for Optimal PCR and qPCR in the Laboratory


 

KEYWORDS: PCR, qPCR, Pipetting, Biomolecule, Disposable Tips, Accuracy, PIPETMAN M, MICROMAN, PIPETMAN L, GmCLab

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Determination of Pesticides in Drinking Water via LC-MS/MS Using Polymeric Solid Phase Extraction Cartridges


 

KEYWORDS: SPE, Pesticide, Herbicide, Fungicide, Pharmaceuticals, Drinking Water, WHO, National Standards

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Efficient LC-MS/MS Method for Determination of delta9-Tetrahydrocannabinol in Human Plasma Using Solid Phase Extraction Clean-up


 

KEYWORDS: SPE, Tetrahydrocannabinol, Metabolites, Human Plasma, LC-MS/MS, LC-MS, GC-MS, MS

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Methamphetamine and Amphetamine Drug Screening in Urine via Solid Phase Extraction


 

KEYWORDS: SPE, Urine Analysis, Drug Screening, mAMP, AMP

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Optimizing Chiral Separations and Purification of Enantiomers through Selective Sample Slicing and Column Switching


 

KEYWORDS: Enantiomers, Purification, Chiral Pharmaceuticals, GX-271 Semi-Preparative HPLC System, Column Switching, Sample Slicing, Sample Cleanup

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Natural Product Purification: Bioactive trans-Cinnamaldehyde from Zingiberaceae Extract for Use as a Skin Lightening Agent


 

KEYWORDS: Cosmetics, Natural Products, Zingiberaceae, trans-Cinnamaldehyde, GX-281 Preparative Purification System, Biologically Active Compounds, High Pressure Liquid Chromatography (HPLC)

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High Loading Purification of a Ribonuclease A Protein Using a Manual Purification System


 

KEYWORDS: Ribonuclease A (RNAse), Protein, Manual HPLC Purification, Bovine Pancreas, Ion Exchange, High Loading Purification

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Purification of a Protein Using the PLC 2020 Personal Purification System


 

KEYWORDS: Protein, Anion Exchange, Purification, Gilson PLC 2020

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Purification of the B-Lactoglobulin A Protein with the Gilson GX-271 LC/MS Purification System


 

KEYWORDS: B-Lactoglobulin, Protein, Mass-Based Purification, Target Ions, Gilson LC/MS System

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Scale-Up of a 6-mer Synthesized Peptide Using a Manual HPLC System


 

KEYWORDS: Peptide, Scale-Up Purification, Gilson Manual System

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Preparative Purification of a Synthesized Peptide Using an Automated LC/MS Purification System


 

KEYWORDS: Peptide, Mass-Based Purification, Target Ions, Gilson LC/MS System

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Purification of a 25-mer ssDNA Oligonucleotide Using the PLC 2020 Personal Purification System


 

KEYWORDS: Single Stranded DNA, Oligonucleotides, Purification, Gilson PLC 2020

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Automated Three-Step Oligonucleotide Purification Using an Automated Preparative HPLC System


 

KEYWORDS: Oligonucleotides, Purification, Reinjection of Fractions, Desalting, Gilson Purification System

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Considerations for Automating the Purification of Monoclonal Antibodies on a Gilson Liquid Handler


 

KEYWORDS: Monoclonal Antibody Purification (mAb), Automated Liquid Handler

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Utilizing a Single Platform GX-281 Liquid Handler to Automate Stability and Solubility Studies for New Drug Pre-formulation


 

KEYWORDS: Drug Formulation, Stability, Solubility, GX-281 Liquid Handler, TRILUTION LH

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Automated NGS Library Prep: Ligation Efficiency of NxSeq® Sample Preparation Using The Gilson PIPETMAX®

 

KEYWORDS: Next Generation Sequencing, NGS, Sample Prep, DNA Library Prep, Automation, NxSeq, PIPETMAX

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Selecting the Appropriate Laboratory Equipment: The Coomassie (Bradford) Protein Assay Performed Manually using Gilson PIPETMAN™ M Multichannel, PIPETMAN L, and Automated using GX-271 ASPEC™ and GX-241 Liquid Handler

KEYWORDS: PIPETMAN® L, PIPETMAN M Multichannel, GX-241 Liquid Handler, GX-274 ASPEC™, Coomassie (Bradford) Protein Assay, Molecular Biology, Automation, Laboratory Equipment

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An Undergraduate's Approach to Method Development: Chromatography Lessons Learned through Experimentation

KEYWORDS: PLC 2020, Purification, Preparative Chromatography, Reversed Phase Chromatography, Method Development, Sparation Science, Pharmaceuticals

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Manual Automation of Rapid N-Glycan Sample Preparation - Reliable Results from Error Free Sample Tracking and Accurate Pipetting

KEYWORDS: N-Glycan, Glycobiology, hlgG, Sample Cleanup, Purification, Glycoanalysis, Microchromatography

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Purification and Analysis of Bioactive Extracts from Marine Yeast with the Gilson Semi-Micro 2D HPLC System

KEYWORDS: 2D HPLC (2 Dimensional High Pressure Liquid Chromatography), TRILUTION LC Liquid Chromatography Software, Gilson 233XL, Bioactive, Marine Yeast

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Automated Separation of Crude Extracts from Natural Products into Fractions for Primary High Throughput Screening

 

KEYWORDS: Gilson 215 Liquid Chromatography System, TRILUTION LC Liquid Chromatography Software, High Throughput Screening, Natural Products

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Natural Product Purification of Lycopene and B-Carotene Using Flash Column Sample Clean-up Prior to High Pressure Normal Phase Chromatography on the Gilson PLC 2020

KEYWORDS: PLC 2020, Natural Product Purification, Flash Chromatography, Lycopene, Tea, B-Carotene, Tomatoes

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A Quick Study - Comparison of Chromatography Normal Phase vs. Flash on the Gilson PLC 2020

KEYWORDS: Purification, Normal Phase Chromatography, Flash Chromatography, PLC 2020, Preparative Chromatography

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Improving Laboratory Efficiency & Flexibility with Automated Sample Preparation Systems - The Impact of the GX-281 Dual Z-Arm Weighing Station

KEYWORDS: Weighing Station, GX-281 Liquid Handler, Sample Preparation, Automated Systems, Bar Coding, Tube Gripper, TRILUTION LH v3.0 Liquid Handling Software

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Automated Solid Phase Extraction (SPE) of Benzodiazepines from Urine using the Gilson GX-274 ASPEC™

KEYWORDS: Gilson GX-274 ASPEC™, TRILUTION® LH, Solid Phase Extraction (SPE), LC/MS-MS Analysis,Diazepam, Benzodiazepines, Urine, Pharmaceuticals

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A Practical User Guide for the Determination of Optimal Purification Gradients for the Gilson PLC 2020

KEYWORDS: Purification, Pharmaceuticals, Optimal Solvent Gradients, Isolation Gradients, Organic Compounds, PLC 2020, Preparative Chromatography

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Automated Determination of VMA, HVA, 5-HIAA, in Urine Using the Gilson GX-274 and Gilson ASPEC™ XL

KEYWORDS: Gilson GX-274, TRILUTION® LH, Catecholamines, Urine, Vanillylmandelic acid (VMA), Homovanilic Acid (HVA), 5-hydroxyindoleacetic Acid (5-HIAA), Tumors, Diagnosis, Biosyntheses, Recipe

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Rapid Isolation of Geraniin from Nephelium Lappaceum Rind Waste Using the Gilson GX-281 Preparative HPLC Purification System

KEYWORDS: Gilson GX-281 Preparative HPLC System, TRILUTION® LC, Geraniin, Alpha Glucosidase, Alpha Amylase, Aldol Reductase, AGE, Nephelium Lappaceum L., University of Malaysia, Anti-Hyperglycemic Agent, Hyperglycemia, Purification, Natural Products, HPLC, Diabetes

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Identifying and Collecting Weak UV Absorbance and Low Level Peaks of Interest From a Complex Mixture Using Conditional Logic Purification on Mass and UV Signals

KEYWORDS: Gilson GX-271 LC/MS Purification System, Fraction Collection, Active Pharmaceutical Ingredient (API), Metoprolol, Verapamil, Chloramphenicol, Doxepin, Sulfamethazine

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Optimizing the Purification of a Standard Chrial Compound Utilizing a Benchtop, Multi-Purpose, Semi-Preparative to Preparative HPLC System

KEYWORDS: Chiral, Enantiomer, HPLC, Normal Phase Chromatography, PLC 2020 Personal Chromatography System, Preparative Chromatography, Purification, Resolution, Trans-Stilbene Oxide

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Automated Solid Phase Extraction of Allantoin from Cosmetics and Topical Pharmaceuticals Prior to Analysis by HPLC 

KEYWORDS: Allantoin, Automation, CHROMABOND HR-XA, Cosmetics Glyoxylic Acid Diureide, GX-271 ASPEC, HILIC, HPLC, Ointments, Pharmaceuticals, Sample Preparation, Sold Phase Extraction, SPE, Topical Pharmaceuticals, 5-Ureidohydantoin

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Processing Human Serum for Rapid and Reproducible N-Glycan Mass Profiling 

KEYWORDS: Automation, Biomarkers, Glycans, Glycomics, GX-274 ASPEC, Human Serum, Mass Profiling, Mass Spectrometry, MALDI-FTICR, MS, N-Glycan, N-linked Glycans, Serum, Solid Phase Extraction, SPE

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FTLC Fraction Trapping Liquid Chromatography

KEYWORDS: HPLC, Preparative Chromotography, Gilson FT-LC System

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Automated Fractionation System that Increases Recovery and Optimizes Precision of Solid Phase Extraction Methodology

KEYWORDS: Solid Phase Extraction, SPE, Serum, Plasma, Urine

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A Novel Approach to GX-281 Semi-Preparative System Purification of Natural Compounds Using Isolation Gradients

KEYWORDS: GX-281 Purification System, TRILUTION LC, natural compounds, Oregano, Rosmarinic Acid, Carvacol, isolation gradient, purification, fraction collection

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Systematic Look at the Affects of Various Solvents, Injection Techniques and Sample Amounts on Preparative HPLC Columns

KEYWORDS: Various Solvents, Injection Techniques, Solvents

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A Totally Automated Solution for Normal and RP Preparative HPLC with Analytical Purification Determination: cLC

KEYWORDS: Analytical Purification Determination, cLC  System, Reverse-Phase (RP) Chromatography, Normal Phase (NP) Chromatography

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A Compact Automated Liquid Handler for Microbatch Protein Crystallography

KEYWORDS: Protein Crystallization, Microbatch

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A Multipurpose Preparative System for the Detection and Collection of Active and Non-active UV/VIS Compounds

KEYWORDS: Active and Non-Active UV/VIS Compounds, ELSD

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An Automated SPE Procedure and Analysis for the Extraction of Chlorpromazine and Thiorizidine from Plasma

KEYWORDS: Neuroleptic Drugs, SPE, SPE 215

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Fast PCLC: A Widely Application Technique for Laboratory Scale Preparative Separations

KEYWORDS: Fast PCLC

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An Automated SPE Procedure and Analysis for the Determination of Ibuprofen and Ketoprofen from Plasma

KEYWORDS: SPE, Ketoprofen, Ibuprofen, Prostaglandin

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The Ephedra Story: An Automated SPE Procedure and Analysis for the Determination of Ephedra and Ephedra-like Compounds in Foods and Dietary Supplements

KEYWORDS: Ephedra, Norephedrine, Pseudoephedrine, Methylephedrine, Dietary Supplements, Nutraceuticals, Gilson SPE 215

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